Identification of Escherichia coli O157 in sheep and goats using PCR technique
نویسندگان
چکیده
منابع مشابه
Identification of Escherichia coli O157: H7 from Slaughtered Beef in Mashhad Using Biochemical and Molecular Methods
Background and Aims: Escherichia coli O157:H7 is one of the main causes of transmitted diseases by food, including meat and meat products. The purpose of this study was to investigate the prevalence of E. coli O157:H7 contamination in Mashhad with the detection of rfbE gene. Materials and Methods: For this study, 148 slaughtered beef samples from Mashhad (spring to winter, 2018) were randomly ...
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data describing the microbiological status of a country’s meat products can be of great value: in acting as a de facto validation of the country’s regulatory systems, as a baseline for setting performance standards and in supporting risk assessment. o26, o45, o91, o103, o111, o113, o121, o128, o145, and o157 are the most common serotypes of enterohemorrhagic escherichia coli associated with hum...
متن کاملDetection of Viable But Non-Culturable State of Escherichia coli O157:H7 Using Reverse Transcription PCR
Background and Aims: Many bacteria including Escherichia coli may enter into a viable but non-culturable (VBNC) state under unfavorable stresses, which are unable to be detected by culture-based methods. In this study, the use of Reverse Transcription PCR (RT-PCR) for detection of VBNC state of E. coli O157:H7 was investigated. Materials and Methods: Escherichia. coli O157:H7 was inoculated i...
متن کاملIsolation and Identification of Pasteurella multocida by PCR from sheep and goats in Fars province, Iran
During one year period from 2010 to 2011 the samples from pneumonic animals were taken and transported to the laboratory. Pasteurella multocida were identified in 16.6% of animal by biochemical test. The high incidence of P. multocida was obtained in the south of Fars province, where the area was warm region. The Mean Death Time between the isolates was 12-18 and 19-24 hours. Only the capsular ...
متن کاملPrevalence and molecular characterization of verotoxin-producing Escherichia coli O157:H7 in cattle and sheep in Shiraz-Iran
Shiga toxin producing Escherichia coli have been associated with HUS, HC and TTP in human. We found recto-anal mucosal sample in sheep as well in cattle is the main site for E. coli O157 localization. 1246 E. coli isolates from 872 both healthy and diarrheic animals were analyzed, by screening for the presence of Shiga toxin-producing (VT 1 and VT 2) and intimin (eae) genes used Multiplex PCR. ...
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ژورنال
عنوان ژورنال: IOSR Journal of Agriculture and Veterinary Science
سال: 2013
ISSN: 2319-2372,2319-2380
DOI: 10.9790/2380-0623032